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Product Name
CCT3 antibody
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Description
CCT3 Mouse Monoclonal antibody. Positive WB detected in COLO 320 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human liver cancer tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 56-60kd
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Tested applications
ELISA, WB, IF, IHC, FC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
CCT gamma antibody; CCT3 antibody; CCTG antibody; hTRiC5 antibody; PIG48 antibody; TCP 1 gamma antibody; TRIC5 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of CCT3 recombinant protein (Accession Number: NM_005998). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107037(CCT3 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells using Catalog No:107037 (CCT3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107037(CCT3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107037(CCT3 Antibody) at dilution of 1:50
1X10^6 HepG2 cells were stained with 0.2ug CCT3 antibody (Catalog No:107037, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
CCT3 represents chaperonin containing TCP1, subunit 3 (gamma)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"