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Product Name
CCR10 antibody
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Description
CCR10 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human spleen tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C C chemokine receptor type 10 antibody; C C CKR 10 antibody; CC CKR 10 antibody; CCR 10 antibody; CCR10 antibody; G protein coupled receptor 2 antibody; GPR2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CCR10 recombinant protein (Accession Number: NM_016602). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109074(CCR10 antibody) at dilution of 1:1000
1X10^6 HepG2 cells were stained with 0.2ug CCR10 antibody (Catalog No:109074, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109074(CCR10 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109074(CCR10 antibody) at dilution of 1:50 (under 40x lens)
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Background
CCR10 (also known as GPR2) is a G-protein coupled receptor for chemokines CCL27 (SCYA27) and CCL28 (SCYA28). CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (PMID: 11821900). CCR10-CCL28 interactions are thought to contribute to the accumulation of IgA Ab-secreting cells (ASC) to mucosal surfaces.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"