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Product Name
CCNT1 Polyclonal Antibody
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Description
Polyclonal antibody to CCNT1
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Tested applications
WB, FC
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Species reactivity
Human, Mouse
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Alternative names
CCNT1 antibody; CCNT antibody; CYCT1 antibody; HIVE1 antibody; cyclin-T1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human CCNT1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:500
FC 1:20 - 1:50 -
Validations
Western blot - CCNT1 Polyclonal Antibody
Western blot analysis of extracts of K-562 cells, using CCNT1 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).; (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"