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CCM3/PDCD10 antibody

Cat.#: 109067

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Product Information

  • Product Name
    CCM3/PDCD10 antibody
  • Documents
  • Description
    CCM3/PDCD10 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HeLa cells, rat skin tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human colon cancer tissue, human liver cancer tissue. Positive IF detected in mouse brain. Observed molecular weight by Western-blot: 25-30 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CCM3 antibody; PDCD10 antibody; programmed cell death 10 antibody; TFAR15 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CCM3/PDCD10 recombinant protein (Accession Number: XM_006713485). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:100-1:500

  • Validations

    WB and IF result of CCM3 antibody (courtesy of Dr. Shah, University of Muenster). IF note: E15 mouse brain cortex was PFA fixed, the sections were antigen retrieved with Na-citrate buffer (pH 6) and blocked with NGS and Triton X100. 1:150 dilution of CCM3 antibody was used with overnight incubation of sections at 4 degrees. Secondary anti-Rabbit conjugated to AF488 was used to detect the staining.

    WB and IF result of CCM3 antibody (courtesy of Dr. Shah, University of Muenster). IF note: E15 mouse brain cortex was PFA fixed, the sections were antigen retrieved with Na-citrate buffer (pH 6) and blocked with NGS and Triton X100. 1:150 dilution of CCM3 antibody was used with overnight incubation of sections at 4 degrees. Secondary anti-Rabbit conjugated to AF488 was used to detect the staining.

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109067(PDCD10 antibody) at dilution of 1:300

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109067(PDCD10 antibody) at dilution of 1:300

    IP Result of anti-PDCD10 (IP:Catalog No:109067, 3ug; Detection:Catalog No:109067 1:300) with mouse skeletal muscle tissue lysate 7500ug.

    IP Result of anti-PDCD10 (IP:Catalog No:109067, 3ug; Detection:Catalog No:109067 1:300) with mouse skeletal muscle tissue lysate 7500ug.

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109067(PDCD10 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109067(PDCD10 antibody) at dilution of 1:50 (under 10x lens)

  • Background
    PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.
  • References
    • Fidalgo M, Fraile M, Pires A, Force T, Pombo C, Zalvide J. CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation. Journal of cell science. 123(Pt 8):1274-84. 2010.
    • Lin C, Meng S, Zhu T, Wang X. PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival. The Journal of biological chemistry. 285(53):41675-85. 2010.
    • Chan AC, Drakos SG, Ruiz OE. Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice. The Journal of clinical investigation. 121(5):1871-81. 2011.
    • Fidalgo M, Guerrero A, Fraile M, Iglesias C, Pombo CM, Zalvide J. Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress. The Journal of biological chemistry. 287(14):11556-65. 2012.
    • Louvi A, Nishimura S, Günel M. Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration. Development (Cambridge, England). 141(6):1404-15. 2014.
    • Fuller SJ, McGuffin LJ, Marshall AK. A novel non-canonical mechanism of regulation of MST3 (mammalian Sterile20-related kinase 3). The Biochemical journal. 442(3):595-610. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"