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Product Name
CCL3 Polyclonal Antibody
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Description
Polyclonal antibody to CCL3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCL3 antibody; G0S19-1 antibody; LD78ALPHA antibody; MIP-1-alpha antibody; MIP1A antibody; SCYA3 antibody; C-C motif chemokine 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human CCL3 (NP_002974.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - CCL3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CCL3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - CCL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using CCL3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCL3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using CCL3 antibody at dilution of 1:100 (40x lens).
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Background
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"