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Product Name
CCL28 antibody
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Description
CCL28 Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue, human colon cancer tissue. Positive WB detected in human spleen tissue, Jurkat cells, PC-3 cells. Observed molecular weight by Western-blot: 8kd-9kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C C motif chemokine 28 antibody; CCK1 antibody; CCL28 antibody; MEC antibody; Protein CCK1 antibody; SCYA28 antibody; Small inducible cytokine A28 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CCL28 recombinant protein (Accession Number: NM_001301874). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:109004(CCL28 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon using Catalog No:109004(CCL28 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:109004(CCL28 antibody) at dilution of 1:50 (under 40x lens)
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Background
CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).
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