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Product Name
CCL11 Polyclonal Antibody
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Description
Polyclonal antibody to CCL11
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCL11 antibody; SCYA11 antibody; eotaxin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human CCL11 (NP_002977.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CCL11 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CCL11 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - CCL11 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using CCL11 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCL11 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CCL11 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCL11 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using CCL11 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CCL11 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using CCL11 antibody . Blue: DAPI for nuclear staining.
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Background
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"