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Product Name
CCDC59 Polyclonal Antibody
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Description
Polyclonal antibody to CCDC59
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCDC59 antibody; BR22 antibody; HSPC128 antibody; TAP26 antibody; coiled-coil domain containing 59 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human CCDC59 (NP_054886.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CCDC59 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CCDC59 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCDC59 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human uterine cancer using CCDC59 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CCDC59 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using CCDC59 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CCDC59 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Component of the transcription complexes of the pulmonary surfactant-associated protein-B (SFTPB) and -C (SFTPC). Enhances homeobox protein Nkx-2.1-activated SFTPB and SFTPC promoter activities.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"