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Product Name
CBX8 Polyclonal Antibody
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Description
Polyclonal antibody to CBX8
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CBX8 antibody; PC3 antibody; RC1 antibody; chromobox 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human CBX8 (NP_065700.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - CBX8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CBX8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CBX8 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CBX8 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat pancreas using CBX8 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using CBX8 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using CBX8 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CBX8 Antibody at dilution of 1:100 (40x lens).
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Background
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"