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Product Name
CBX6 Polyclonal Antibody
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Description
Polyclonal antibody to CBX6
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CBX6 antibody; chromobox 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-180 of human CBX6 (NP_055107.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CBX6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CBX6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using CBX6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CBX6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CBX6 antibody at dilution of 1:100 (40x lens).
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Background
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin. Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"