CATHPb1 peptide

CATHPb1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-KRFKKFFRKIKKGFRKIFKKTKIFIGGTIPI-OH

H-Lys-Arg-Phe-Lys-Lys-Phe-Phe-Arg-Lys-Ile-Lys-Lys-Gly-Phe-Arg-Lys-Ile-Phe-Lys-Lys-Thr-Lys-Ile-Phe-Ile-Gly-Gly-Thr-Ile-Pro-Ile-OH

31

95.2%

C₁₈₇H₃₀₈N₅₀O₃₄

3800.75

Synthetic

Powder

Python Cathelicidin CATHPb1 has protective effect in a broad range of in vivo infection models, especially MRSA and VRSA mice model, by local and systemic administration. CATHPb1 was not only directly antimicrobial and anti-biofilm in vitro, but also immunomodulatory in vivo. The protection was all effective through different administration routes, but was blocked by in vivo depletion of monocyte/macrophages or neutrophils. CATHPb1 can rapidly and massively modulate macrophages/monocytes and neutrophils trafficking to the infection site, and potentiate their bactericidal functions. Meanwhile, CATHPb1 remarkably augmented neutrophil-mediated bacteria killing by facilitating neutrophil extracellular traps (NETs) formation and preventing its degradation. Acting through MAPKs and NF-κB pathways, CATHPb1 selectively enhanced the levels of chemokines while reducing the production of pro-inflammatory cytokines without undesirable toxicities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Cai S, Qiao X, Feng L, et al. Python Cathelicidin CATHPb1 Protects against Multidrug-Resistant Staphylococcal Infections by Antimicrobial-Immunomodulatory Duality. J Med Chem. 2018;61(5):2075-2086

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.