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Product Name
Cathepsin H antibody
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Description
Cathepsin H Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in SH-SY5Y cells, HepG2 cells, human lung tissue. Observed molecular weight by Western-blot: 41 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACC 4 antibody; ACC 5 antibody; Cathepsin H antibody; CPSB antibody; CTSH antibody; DKFZp686B24257 antibody; minichain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cathepsin H recombinant protein (Accession Number: NM_004390). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:108892(CTSH antibody) at dilution of 1:50 (under 10x lens)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:108892(CTSH Antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:108892(CTSH Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cathepsin H (CTSH, synonyms: CPSB, MGC1519, minichain) is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.
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References
- Wu SM, Huang YH, Yeh CT. Cathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cells. Oncogene. 30(17):2057-69. 2011.
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