Cathepsin B antibody

Cat.#: 108887

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Product Information

  • Product Name
    Cathepsin B antibody
  • Documents
  • Description
    Cathepsin B Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue, human ovary tumor tissue. Positive IF detected in A375 cells. Positive WB detected in A375 cells, HT-1080 cells, human brain tissue, human liver tissue, mouse brain tissue, NIH/3T3 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 38 kDa, 44 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    APP secretase antibody; APPS antibody; cathepsin B antibody; Cathepsin B1 antibody; CPSB antibody; CTSB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cathepsin B recombinant protein (Accession Number: XM_011543812). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:108887(CTSB antibody) at dilution of 1:800

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:108887(CTSB antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108887(CTSB antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108887(CTSB antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108887(CTSB antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108887(CTSB antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of A375 cells using Catalog No:108887(Cathepsin B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of A375 cells using Catalog No:108887(Cathepsin B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 MCF-7 cells were stained with 0.2ug Cathepsin B antibody (Catalog No:108887, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug Cathepsin B antibody (Catalog No:108887, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa marure forms in mouse brain by western blot(PMID:20616152).
  • References
    • Xiang Y, Yan C, Guo X. Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection. Proceedings of the National Academy of Sciences of the United States of America. 111(18):6702-7. 2014.
    • Yan Y, Zhou K, Wang L, Zhou Y, Chen X, Fan Q. Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma. International journal of clinical and experimental pathology. 8(9):10102-11. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"