Cathelicidin-RC1 peptide

Cathelicidin-RC1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KKCKFFCKVKKKIKSIGFQIPIVSIPFK

Lys-Lys-Cys-Lys-Phe-Phe-Cys-Lys-Val-Lys-Lys-Lys-Ile-Lys-Ser-Ile-Gly-Phe-Gln-Ile-Pro-Ile-Val-Ser-Ile-Pro-Phe-Lys

28

96.5%

Synthetic

Powder

51.35% similar to AP2424 Crotalicidin. More similar to reptile cathelicidin than to other amphibian peptides. Rich in K (32%). Active against Gram- E. coli (MIC 2.8-5.7 uM), S. dysenteriae, K. peneumoniae (5.7-22 uM, >200 for one strain), K. oxytoca (1.43 uM), P. mirabilis (5.7 uM), S. maltophilia (11.4 uM), S. maltophilia, P. aeruginosa ATCC27853 (2.9 uM) (mostly clinical strains), Gram+ bacteria S. aureus (3 active MIC 2.9 uM; 3 inactive MIC>200 uM), B. subtilis, S. epidermidis, E. faecium (2.9 uM), N. asteroides, E. faecalis (MIC 22.8 uM), and fungi C. albicans (clinical strains, MIC 2.9 to >200 uM). Activity against E. coli ATCC25922 is salt-sensitive (4.7 uM no salt, 4.7 uM at 100 uM NaCl, 18.8 uM at 400 mM NaCl). Cathelicidin-RC1 was found in the American bullfrog Rana catesbeiana , North America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Guo C, Hu Y, Li J, et al. Identification of multiple peptides with antioxidant and antimicrobial activities from skin and its secretions of Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus. Biochimie. 2014;105:192-201.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.