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Product Name
Catalase-Specific antibody
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Description
Catalase-Specific Rabbit Polyclonal antibody. Positive WB detected in HT-1080 cells, HeLa cells. Positive IHC detected in human hepat℃irrhosis tissue. Positive IF detected in Hela cells, HepG2 cells. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CAT antibody; Catalase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001752). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:108885(CAT-Specific antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:108885(CAT-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:108885(CAT-Specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CAT antibody Catalog No:108885 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia. The antibody is specific to CAT.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"