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Product Name
Caspase 12 antibody
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Description
Caspase 12 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue. Positive WB detected in HEK-293 cells, HepG2 cells, Jurkat cells, L02 cells, mouse heart tissue, mouse lung tissue, NIH/3T3 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 36-42 kDa, 50 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CASP 12 antibody; CASP12 antibody; CASP12P1 antibody; caspase 12 (gene/pseudogene) antibody; Caspase12 antibody; Inactive caspase 12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NR_000035). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108872(CASP12 antibody) at dilution of 1:1000
WB result of Catalog No:108872 (CASP12 antibody) with various lysates.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:108872(Caspase 12 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:108872(Caspase 12 Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Caspase 12 (IP:Catalog No:108872, 4ug; Detection:Catalog No:108872 1:1000) with HEK-293 cells lysate 2000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:108872(Caspase 12 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.
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References
- Zhang S, Wang C, Tang S. Inhibition of autophagy promotes caspase-mediated apoptosis by tunicamycin in HepG2 cells. Toxicology mechanisms and methods. 24(9):654-65. 2014.
- Huang Y, Du Y, Zhang X. Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 70:146-50. 2015.
- Xu D, Su C, Song X. Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release. Chemical research in toxicology. 28(6):1326-37. 2015.
- Zhou GZ, Cao FK, Du SW. The apoptotic pathways in the curcumin analog MHMD-induced lung cancer cell death and the essential role of actin polymerization during apoptosis. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 71:128-34. 2015.
- Koubkova L, Vyzula R, Karban J. Evaluation of cytotoxic activity of titanocene difluorides and determination of their mechanism of action in ovarian cancer cells. Investigational new drugs. 33(5):1123-32. 2015.
- Wang C, Li T, Tang S. Thapsigargin induces apoptosis when autophagy is inhibited in HepG2 cells and both processes are regulated by ROS-dependent pathway. Environmental toxicology and pharmacology. 41:167-79. 2016.
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