-
Product Name
CASP5 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CASP5
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
CASP5 antibody; ICE(rel)III antibody; ICEREL-III antibody; ICH-3 antibody; caspase-5 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-250 of human CASP5 (NP_001129584).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - CASP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CASP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
-
Background
Mediator of programmed cell death (apoptosis). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"