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Product Name
CASP12 Polyclonal Antibody
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Description
Polyclonal antibody to CASP12
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CASP12 antibody; CASP-12 antibody; CASP12P1 antibody; inactive caspase-12 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP12 (NP_001177945.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CASP12 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CASP12 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CASP12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using CASP12 antibody at dilution of 1:100 (20x lens).
Immunohistochemistry - CASP12 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using CASP12 antibody at dilution of 1:100 (40x lens).
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Background
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"