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Product Name
CASK Polyclonal Antibody
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Description
Polyclonal antibody to CASK
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CASK antibody; CAGH39 antibody; CAMGUK antibody; CMG antibody; FGS4 antibody; LIN2 antibody; MICPCH antibody; MRXSNA antibody; TNRC8 antibody; peripheral plasma membrane protein CASK antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASK (NP_001119526.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CASK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CASK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - CASK Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CASK antibody . Blue: DAPI for nuclear staining.
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Background
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"