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Product Name
Casein Kinase 2 beta antibody
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Description
Casein Kinase 2 beta Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human breast cancer tissue, mouse testis tissue. Positive FC detected in HeLa cells. Positive WB detected in MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 25-30 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Casein kinase II subunit beta antibody; CK II beta antibody; CK2B antibody; CK2N antibody; CSK2B antibody; CSNK2B antibody; G5A antibody; Phosvitin antibody; Protein G5a antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Casein Kinase 2 beta recombinant protein (Accession Number: NM_001320). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108867(CSNK2B Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:108867(CSNK2B Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:108867(CSNK2B Antibody) at dilution of 1:50
1X10^6 HeLa cells were stained with 0.2ug CSNK2B antibody (Catalog No:108867, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Casein kinase II subunit beta is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.
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