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Product Name
Casein Kinase 1 delta antibody
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Description
Casein Kinase 1 delta Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 47kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Casein Kinase 1 delta antibody; casein kinase 1 antibody; delta antibody; Casein kinase I isoform delta antibody; CKI delta antibody; CKId antibody; CSNK1D antibody; HCKID antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Casein Kinase 1 delta recombinant protein (Accession Number: NM_001893). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108865(CSNK1D antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells, using CSNK1D antibody Catalog No:108865 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CSNK1D (IP:Catalog No:108865, 3ug; Detection:Catalog No:108865 1:1000) with HeLa cells lysate 2000ug.
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Background
CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.
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References
- Chen H, Ma H, Inuzuka H. DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase. Molecular and cellular biology. 33(6):1139-48. 2013.
- Qi ST, Wang ZB, Huang L. Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression. Cell cycle (Georgetown, Tex.). 14(11):1675-85. 2015.
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