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Product Name
CAS9 Polyclonal Antibody
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Description
Polyclonal antibody to CAS9
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Tested applications
WB, IF
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Species reactivity
Streptococcus pyogenes
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of streptococcus pyogenes CAS9
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:5000 - 1:10000
IF 1:50 - 1:200 -
Validations
Western blot - CAS9 Polyclonal Antibody
Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - CAS9 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) use CAS9 Rabbit pAb (red, ) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"