-
Product Name
CARD11 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CARD11
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
CARD11 antibody; BENTA antibody; BIMP3 antibody; CARMA1 antibody; IMD11 antibody; PPBL antibody; caspase recruitment domain family member 11 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - CARD11 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CARD11 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - CARD11 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using CARD11 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - CARD11 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using CARD11 antibody . Blue: DAPI for nuclear staining.
-
Background
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"