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Product Name
CARD10 Polyclonal Antibody
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Description
Polyclonal antibody to CARD10
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
CARD10 antibody; BIMP1 antibody; CARMA3 antibody; caspase recruitment domain family member 10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 863-1032 of human CARD10 (NP_055365.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - CARD10 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CARD10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - CARD10 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using CARD10 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CARD10 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using CARD10 antibody at dilution of 1:100 (40x lens).
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Background
Activates NF-kappa-B via BCL10 and IKK.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"