Mouse Carboxypeptidase B1/CPB1 (His Tag) recombinant protein

Mouse Carboxypeptidase B1/CPB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Carboxypeptidase B1 (Tissue)

AI504870; CPB1; DKFZP779K1333; CPB; 2210008M23RIK; 0910001A18RIK; CARBOXYPEPTIDASE B1; 1810063F02RIK; PASP; PCPB

B2RS76

Cpb1; mCG_21890

Human Cells

A DNA sequence encoding the mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse CPB1 comprises 411 amino acids with a predicted molecular mass of 47.5 kDa. The apparent molecular mass of rm CPB1 is approximately 43-45 kDa in SDS-PAGE under reducing conditions.

> 94 % as determined by SDS-PAGE

Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >5,000 pmoles/min/μg.