Mouse Carboxypeptidase A1/CPA1 (His Tag) recombinant protein
Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Protein short names
CPA1; CPA; CARBOXYPEPTIDASE A1; 0910001L12RIK
A DNA sequence encoding the mouse CPA1 (NP_079626.2) (Met 1-Tyr 419) precusor was expressed with a C-terminal polyhistidine tag.
The secreted recombinant pro form of mouse CPA1 comprises 414 amino acids and has a calculated molecular mass of 47 kDa. The recombinant protein migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
> 96 % as determined by SDS-PAGE
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >6,000 pmoles/min/μg.
Mouse Carboxypeptidase A1 / CPA1 Protein (His Tag) SDS-PAGE
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