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Product Name
CAPN3 Polyclonal Antibody
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Description
Polyclonal antibody to CAPN3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CAPN3 antibody; CANP3 antibody; CANPL3 antibody; LGMD2 antibody; LGMD2A antibody; nCL-1 antibody; p94 antibody; calpain-3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-309 of human CAPN3 (NP_775111.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CAPN3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CAPN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Calcium-regulated non-lysosomal thiol-protease.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"