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Product Name
Calpastatin antibody
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Description
Calpastatin Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human colon cancer tissue, human skeletal muscle tissue. Positive IF detected in HeLa cells. Positive IP detected in K-562 cells. Positive WB detected in HeLa cells, K-562 cells, MCF7 cells. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
BS 17 antibody; Calpain inhibitor antibody; calpastatin antibody; CAST antibody; Sperm BS 17 component antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Calpastatin recombinant protein (Accession Number: NM_001042444). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108904(CAST antibody) at dilution of 1:1000
IP Result of anti-CAST (IP:Catalog No:108904, 4ug; Detection:Catalog No:108904 1:1000) with K-562 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108904(CAST Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108904(CAST Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:108904(CAST Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
CAST represents calpastatin
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References
- An K, Fang L, Luo R. Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway. Journal of proteome research. 13(12):5376-90. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"