-
Product Name
Calmegin antibody
- Documents
-
Description
Calmegin Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue. Observed molecular weight by Western-blot: 93 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
calmegin antibody; CLGN antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Calmegin recombinant protein (Accession Number: NM_004362). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108825(CLGN antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108825(CLGN Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108825(CLGN Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-CLGN (IP:Catalog No:108825, 4ug; Detection:Catalog No:108825 1:1000) with mouse testis tissue lysate 4000ug.
-
Background
Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"