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Product Name
Caldesmon antibody
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Description
Caldesmon Rabbit Polyclonal antibody. Positive WB detected in mouse small intestine tissue, HepG2 cells, mouse spleen tissue, PC-3 cells. Positive IHC detected in human colon tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 61-65kd,70-80kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CAD antibody; CALD1 antibody; Caldesmon antibody; caldesmon 1 antibody; CDM antibody; H CAD antibody; L CAD antibody; NAG22 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Caldesmon recombinant protein (Accession Number: NM_004342). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:108821(CALD1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using CALD1 antibody Catalog No:108821 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:108821(CALD1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:108821(CALD1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
CALD1 represents caldesmon 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"