Human CaM Kinase 4/CaMKIV (GST Tag) recombinant protein

Human CaM Kinase 4/CaMKIV (GST Tag) recombinant protein

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Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

Calcium/calmodulin-dependent protein kinase type IV

CAMK4; CAMK; CAMKIV/GR; A430110E23RIK; CAMK-GR; D18BWG0362E; CAMKIV; AI666733; CAMK IV; MGC36771; IV

Q16566

CAMK4; CAMK; CAMK-GR; CAMKIV

Baculovirus-Insect Cells

A DNA sequence encoding the human CAMK4 (NP_001735.1) (Met 1-Tyr 473) was fused with the GST tag at the N-terminus.

Human

The recombinant human CAMK4/GST chimera consists of 697 amino acids and has a predicted molecular mass of 79 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.

> 82 % as determined by SDS-PAGE

No Kinase Activity