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Product Name
CACYBP antibody
- Documents
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Description
CACYBP Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, human skin tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CACYBP antibody; calcyclin binding protein antibody; GIG5 antibody; hCacyBP antibody; PNAS 107 antibody; RP1 102G20.6 antibody; S100A6 binding protein antibody; S100A6BP antibody; Siah interacting protein antibody; SIP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CACYBP recombinant protein (Accession Number: NM_014412). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108770(CACYBP antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:108770(CACYBP antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CACYBP antibody Catalog No:108770 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-CACYBP (IP:Catalog No:108770, 4ug; Detection:Catalog No:108770 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug CACYBP antibody (Catalog No:108770, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exsit as a dimer(PMID:17916393).
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