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Product Name
CACNG4 Polyclonal Antibody
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Description
Polyclonal antibody to CACNG4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CACNG4 antibody; voltage-dependent calcium channel gamma-4 subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 208-327 of human CACNG4 (NP_055220.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CACNG4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CACNG4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - CACNG4 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using CACNG4 antibody . Blue: DAPI for nuclear staining.
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Background
Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit. Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs), including GRIA1 and GRIA4. Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"