-
Product Name
CA9 antibody
- Documents
-
Description
CA9 Mouse Monoclonal antibody. Positive WB detected in human stomach tissue, pig brain tissue, pig stomach tissue. Positive IF detected in HEK-293 cells. Positive IHC detected in human renal cell carcinoma tissue. Observed molecular weight by Western-blot: 50 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse, Pig; other species not tested.
-
Alternative names
CA IX antibody; CA9 antibody; CAIX antibody; Carbonate dehydratase IX antibody; Carbonic anhydrase 9 antibody; carbonic anhydrase IX antibody; G250 antibody; Membrane antigen MN antibody; P54/58N antibody; pMW1 antibody; RCC assOCiated antigen G250 antibody
-
Isotype
Mouse IgG2a
-
Preparation
This antibody was obtained by immunization of CA9 recombinant protein (Accession Number: NM_001216). Purification method: Protein G purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:107030(CA9 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:107030(CA9 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:107030(CA9 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HEK-293 cells using Catalog No:107030(CA9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
CA9 represents carbonic anhydrase IX
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"