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CA3 antibody

Cat.#: 108748

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Product Information

  • Product Name
    CA3 antibody
  • Documents
  • Description
    CA3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, mouse skeletal muscle tissue. Positive IHC detected in human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CA III antibody; CA3 antibody; CAIII antibody; Car3 antibody; Carbonate dehydratase III antibody; Carbonic anhydrase 3 antibody; Carbonic anhydrase III antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CA3 recombinant protein (Accession Number: NM_005181). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108748(CA3 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108748(CA3 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108748(CA3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108748(CA3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108748(CA3 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:108748(CA3 antibody) at dilution of 1:100 (under 40x lens)

  • Background
    Carbonic anhydrase III (CA3) is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase.(PMID:10064618). This protein belongs to the alpha-carbonic anhydrase family.
  • References
    • Wang Y, Kou Y, Wang X, Cederbaum A, Wang R. Multifactorial comparative proteomic study of cytochrome P450 2E1 function in chronic alcohol administration. PloS one. 9(3):e92504. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"