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Product Name
CA2 antibody
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Description
CA2 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse ovary tissue, human stomach tissue, rat kidney tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CA II antibody; CA2 antibody; CAC antibody; CAII antibody; Car2 antibody; Carbonate dehydratase II antibody; Carbonic anhydrase 2 antibody; Carbonic anhydrase C antibody; carbonic anhydrase II antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CA2 recombinant protein (Accession Number: NM_000067). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:108747(CA2 antibody) at dilution of 1:500
IP Result of anti-CA2 (IP:Catalog No:108747, 4ug; Detection:Catalog No:108747 1:500) with mouse kidney tissue lysate 4000ug.
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Background
CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).
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References
- Khositseth S, Pisitkun T, Slentz DH. Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells. Molecular & cellular proteomics : MCP. 10(1):M110.004036. 2011.
- Hong JH, Muhammad E, Zheng C. Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3 (-) secretion revealed by disease causing human mutation. The Journal of physiology. 593(24):5299-312. 2015.
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