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Product Name
C9orf72 antibody
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Description
C9orf72 Mouse Monoclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in SH-SY5Y cells. Positive IP detected in C6 cells. Positive WB detected in human brain tissue, C6 cells, Neuro-2a cells, Y79 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of C9orf72 recombinant protein (Accession Number: BC020851). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107029(C9orf72 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:107029(C9orf72 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:107029(C9orf72 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:107029(C9orf72 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
IP Result of anti-C9orf72 (IP:Catalog No:107029, 4ug; Detection:Catalog No:107029 1:1000) with C6 cells lysate 1320ug.
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Background
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. C9ORF72 has two isoforms with MW 54-60 kDa and 25-30 kDa.
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