C4orf14 antibody

Cat.#: 108730

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Product Information

  • Product Name
    C4orf14 antibody
  • Documents
  • Description
    C4orf14 Rabbit Polyclonal antibody. Positive IHC detected in human colon. Positive WB detected in mouse kidney tissue, mouse brain tissue. Observed molecular weight by Western-blot: 85-95 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C4orf14 antibody; hAtNOS1 antibody; mAtNOS1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of C4orf14 recombinant protein (Accession Number: NM_032313). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:108730(C4orf14 antibody) at dilution of 1:1500

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:108730(C4orf14 antibody) at dilution of 1:1500

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108730(C4orf14 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108730(C4orf14 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108730(C4orf14 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon using Catalog No:108730(C4orf14 antibody) at dilution of 1:100 (under 40x lens)

  • Background
    Nitric oxide associated-1 (NOA1), also known as C4orf14, is an evolutionarily conserved guanosine triphosphate (GTP) binding protein. It localizes predominantly to mitochondria in mammalian cells. Human NOA1 has been shown to interact with complex I of the electron transport chain and with three mitoribosomal proteins, MRPL12, MRPS27, and MRPS29, indicating that NOA1 may interact with the mitoribosome. Recent studies suggest that C4orf14 interacts with human mitochondrial nucleoids, and is involved in the biogenesis of the small mitochondrial ribosomal subunit.

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