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Product Name
C1QB Polyclonal Antibody
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Description
Polyclonal antibody to C1QB
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Tested applications
WB, FC
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Species reactivity
Human, Mouse
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Alternative names
C1QB antibody; complement C1q B chain antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human C1QB
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
FC 1:20 - 1:50 -
Validations
Western blot - C1QB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using C1QB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"