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Product Name
C1GALT1C1 Polyclonal Antibody
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Description
Polyclonal antibody to C1GALT1C1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
C1GALT1C1 antibody; C1GALT2 antibody; C38H2-L1 antibody; COSMC antibody; HSPC067 antibody; MST143 antibody; TNPS antibody; C1GALT1-specific chaperone 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human C1GALT1C1 (NP_689905.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - C1GALT1C1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using C1GALT1C1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - C1GALT1C1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using C1GALT1C1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - C1GALT1C1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using C1GALT1C1 antibody at dilution of 1:100 (40x lens).
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Background
Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"