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Product Name
C14orf138 antibody
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Description
C14orf138 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in PC-3 cells, HepG2 cells, human testis tissue. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
C14orf138 antibody; FLJ13920 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of C14orf138 recombinant protein (Accession Number: BC105118). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108658(C14orf138 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108658(C14orf138 Antibody) at dilution of 1:50 (under 40x lens)
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Background
C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.
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