Product Name

c-MYC antibody

Documents

Description

c-MYC Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue, human breast cancer tissue, human prostate cancer tissue. Positive IP detected in HL-60 cells. Positive WB detected in Raji cells, HL-60 cells, mouse embryo tissue. Observed molecular weight by Western-blot: 62-65 kDa, 50 kDa

Tested applications

ELISA, WB, IP, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

bHLHe39 antibody; c Myc antibody; c-Myc antibody; MRTL antibody; MYC antibody; Myc proto oncogene protein antibody; Proto oncogene c Myc antibody; Transcription factor p64 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of c-MYC recombinant protein (Accession Number: NM_002467). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109418(MYC antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109418(MYC antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-MYC (IP:Catalog No:109418, 3ug; Detection:Catalog No:109418 1:500) with HL-60 cells lysate 1200ug.

Background

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186]

References

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Aifuwa I, Giri A, Longe N, Lee SH, An SS, Wirtz D. Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility. Oncotarget. 6(31):30516-31. 2015.
Fan Z, Cui H, Xu X. MiR-125a suppresses tumor growth, invasion and metastasis in cervical cancer by targeting STAT3. Oncotarget. 6(28):25266-80. 2015.
Wang Y, Jiang L, Ji X, Yang B, Zhang Y, Fu XD. Hepatitis B viral RNA directly mediates down-regulation of the tumor suppressor microRNA miR-15a/miR-16-1 in hepatocytes. The Journal of biological chemistry. 288(25):18484-93. 2013.
Xiang J, Fu X, Ran W. Expression and role of grainyhead-like 2 in gastric cancer. Medical oncology (Northwood, London, England). 30(4):714. 2013.
Liao XH, Wang N, Liu LY. MRTF-A and STAT3 synergistically promote breast cancer cell migration. Cellular signalling. 26(11):2370-80. 2014.

c-MYC antibody

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