-
Product Name
BUB3 antibody
- Documents
-
Description
BUB3 Rabbit Polyclonal antibody. Positive IF detected in HepG2.
-
Tested applications
ELISA, IF
-
Species reactivity
human, mouse, rat; other species not tested.
-
Alternative names
BUB3 antibody; hBUB3 antibody; BUB3L antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BUB3 recombinant protein (Accession Number: NM_004725). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
IF: 1:20-1:200
-
Validations
Immunofluorescent analysis of HepG2 cells, using BUB3 antibody Catalog No:108552 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
BUB3 is a conserved component of the mitotic spindle assembly complex and is an essential compent of spindle-assembly checkpoint (SAC) signaling that operates during early embryogenesis. BUB3 null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. In mitosis, the SAC prevents anaphase onset until all chromosomes have been attached to the spindle microtubules and aligned correctly at the equatorial metaphase plate. BUB3 is involved in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments in mammalian oocyte meiosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"