BTM-P1 peptide

BTM-P1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

VAPIAKYLATALAKWALKQGFAKLKS

Val-Ala-Pro-Ile-Ala-Lys-Tyr-Leu-Ala-Thr-Ala-Leu-Ala-Lys-Trp-Ala-Leu-Lys-Gln-Gly-Phe-Ala-Lys-Leu-Lys-Ser

26

95.5%

C₁₃₄H₂₁₉N₃₃O₃₁

2788.44

Synthetic

Powder

~42% similar to AP1959: frog Temporin-HN1. Artificially synthesized based on the bacterial Cry11Bb protein, which is toxic to mosquito larvae. The sequence has been patented. At 7.1 uM, this synthetic peptide inhibited the growth of E. coli ATCC 25922, S. aureus ATCC 25923, S. pneumoniae ATCC 49619, H. influenzae ATCC 49247, E. faecalis ATCC 29212, P. aeruginosa ATCC27603, P. aeruginosa (Multi-Resistant), and K. pneumoniae ATCC70603 (Segura C et al., 2007). In addition, the protease-resistant BTM-P1d (i.e., synthesized using D-amino acids), not the BTM-P1 synthesized in L-amino acids, displayed A. aegypti larval toxicity (Lemeshko VV, Orduz S., 2013). It adopts a helical conformation in 30% TFE. MOA: it appears to permeate membranes. Deposited and provided by Sergio Orduz on May 6, 2014. APD release May 8, 2014. The source of BTM-P1 is Synthetic.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lemeshko VV, Arias M, Orduz S. Mitochondria permeabilization by a novel polycation peptide BTM-P1. J Biol Chem. 2005;280(16):15579-86.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.