-
Product Name
BTAF1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to BTAF1
-
Tested applications
WB, IHC, IP, ChIP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
BTAF1 antibody; MOT1 antibody; TAF170 antibody; TAF172 antibody; TAFII170 antibody; TATA-binding protein-associated factor 172 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:100 -
Validations
Western blot - BTAF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BTAF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunoprecipitation - BTAF1 Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody . Western blot was performed from the immunoprecipitate using BTAF1 antibody at a dilition of 1:500.
Chromatin Immunoprecipitation - BTAF1 Polyclonal Antibody
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
-
Background
Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"