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Product Name
BST2 antibody
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Description
BST2 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human liver tissue, L02 cells. Positive FC detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 30-36 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
Bone marrow stromal antigen 2 antibody; BST 2 antibody; BST2 antibody; CD317 antibody; HM1.24 antigen antibody; Tetherin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BST2 recombinant protein (Accession Number: NM_004335). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108534(BST2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:108534(BST2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:108534(BST2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:108534(BST2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug BST2 antibody (Catalog No:108534, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection.
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References
- Yan R, Zhao X, Cai D. The Interferon-Inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion. Journal of virology. 89(18):9200-12. 2015.
- Weidner JM, Jiang D, Pan XB, Chang J, Block TM, Guo JT. Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms. Journal of virology. 84(24):12646-57. 2010.
- Pan XB, Han JC, Cong X, Wei L. BST2/tetherin inhibits dengue virus release from human hepatoma cells. PloS one. 7(12):e51033. 2012.
- Pan XB, Qu XW, Jiang D, Zhao XL, Han JC, Wei L. BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells. Antiviral research. 98(1):54-60. 2013.
- Zhang W, Zhang L, Wu Z, Tien P. Differential interferon pathway gene expression patterns in Rhabdomyosarcoma cells during Enterovirus 71 or Coxsackievirus A16 infection. Biochemical and biophysical research communications. 447(3):550-5. 2014.
- Zhao X, Guo F, Liu F. Interferon induction of IFITM proteins promotes infection by human coronavirus OC43. Proceedings of the National Academy of Sciences of the United States of America. 111(18):6756-61. 2014.
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