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Product Name
BSND Polyclonal Antibody
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Description
Polyclonal antibody to BSND
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
BSND antibody; BART antibody; DFNB73 antibody; barttin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - BSND Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BSND antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry - BSND Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using BSND antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BSND Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using BSND antibody at dilution of 1:100 (40x lens).
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Background
Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"