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Product Name
BRN2-Specific antibody
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Description
BRN2-Specific Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A375 cells, HEK-293 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 47kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Brain 2 antibody; Brn 2 antibody; BRN2 antibody; OCt 7 antibody; OCT7 antibody; OCtamer binding protein 7 antibody; OTF 7 antibody; OTF7 antibody; POU class 3 homeobox 2 antibody; POU3F2 antibody; POUF3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005604). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117242(POU3F2-Specific antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human brain using Catalog No:117242(POU3F2-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:117242(POU3F2-Specific antibody) at dilution of 1:100 (under 40x lens)
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Background
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.
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