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Product Name
BRF2 antibody
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Description
BRF2 Rabbit Polyclonal antibody. Positive WB detected in HT-1080 cells, DU 145 cells. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B related factor 2 antibody; BRF 2 antibody; BRF2 antibody; BRFU antibody; FLJ11052 antibody; hBRFU antibody; hTFIIIB50 antibody; TFIIIB50 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRF2 recombinant protein (Accession Number: NM_018310). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:117234(BRF2 antibody) at dilution of 1:300
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Background
Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.
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