Brevinin-2LF2 peptide
Not For Human Use, Lab Use Only.
Cat.#: 300676
Special Price 527.4 USD
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Product Name
Brevinin-2LF2 peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-SIMSTLKQFGISAIKGAAQNVLGVLSCKIAKTC-NH2
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Sequence
H-Ser-Ile-Met-Ser-Thr-Leu-Lys-Gln-Phe-Gly-Ile-Ser-Ala-Ile-Lys-Gly-Ala-Ala-Gln-Asn-Val-Leu-Gly-Val-Leu-Ser-Cys-Lys-Ile-Ala-Lys-Thr-Cys-NH2
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Length (aa)
33
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Peptide Purity (HPLC)
95.84%
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Molecular Formula
C148H259N41O42S3
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Molecular Weight
3381.07
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Source
Synthetic
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Form
Powder
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Description
Brevinin-2LF2 is a peptide identified from the skin secretions of amphibians, specifically Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus.
Brevinin-2LF2 belongs to the brevinin-2 family of antimicrobial peptides, and has 60.6% similarity to Brevinin-2TP1.
Brevinin-2LF2 has been found to exhibit antimicrobial activity against Gram-negative bacterium E. cloacae CGMCC 1.57 (MIC 100 uM), P. faecalis X29 (MIC 6.3 uM), Gram-positive bacterium S. aureus ATCC 25923 (MIC 25 uM), E. faecium 091299 (MIC 25 uM), E. faecalis 981 (MIC 25 uM), and N. asteroides 201118 (MIC 3.1 uM). However, there is no information available regarding its hemolytic activity or other bioactivities. -
Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Guo C, Hu Y, Li J, et al. Identification of multiple peptides with antioxidant and antimicrobial activities from skin and its secretions of Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus. Biochimie. 2014;105:192-201.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"