Brevinin-2LF2 peptide

Brevinin-2LF2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SIMSTLKQFGISAIKGAAQNVLGVLSCKIAKTC-NH2

H-Ser-Ile-Met-Ser-Thr-Leu-Lys-Gln-Phe-Gly-Ile-Ser-Ala-Ile-Lys-Gly-Ala-Ala-Gln-Asn-Val-Leu-Gly-Val-Leu-Ser-Cys-Lys-Ile-Ala-Lys-Thr-Cys-NH2

33

95.84%

C₁₄₈H₂₅₉N₄₁O₄₂S₃

3381.07

Synthetic

Powder

Brevinin-2LF2 is a peptide identified from the skin secretions of amphibians, specifically Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus.
Brevinin-2LF2 belongs to the brevinin-2 family of antimicrobial peptides, and has 60.6% similarity to Brevinin-2TP1.
Brevinin-2LF2 has been found to exhibit antimicrobial activity against Gram-negative bacterium E. cloacae CGMCC 1.57 (MIC 100 uM), P. faecalis X29 (MIC 6.3 uM), Gram-positive bacterium S. aureus ATCC 25923 (MIC 25 uM), E. faecium 091299 (MIC 25 uM), E. faecalis 981 (MIC 25 uM), and N. asteroides 201118 (MIC 3.1 uM). However, there is no information available regarding its hemolytic activity or other bioactivities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Guo C, Hu Y, Li J, et al. Identification of multiple peptides with antioxidant and antimicrobial activities from skin and its secretions of Hylarana taipehensis, Amolops lifanensis, and Amolops granulosus. Biochimie. 2014;105:192-201.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.